Fibrinolysis with Lower Dose Urokinase in Patients with Complicated Parapneumonic Effusion

Fibrinolysis with Lower Dose Urokinase in Patients with Complicated Parapneumonic Effusion

Background: Intrapleural urokinase is one of the most widely used fibrinolytic agents in the treatment of complicated parapneumonic effusion (CPPE). However, there is little research for optimal urokinase doses. The purpose of this study was to evaluate the efficacy of the treatment of half the urokinase dose compared to conventional doses.

Method: We retrospectively register 92 patients with CPPE or Empyema undergoing intrapleural urokinase treatment in 2 tertiary hospitals. Patients receive antibiotics, chest tube drainage, and other treatments as part of routine maintenance. The main results are the level of success of treatment in half the dose of urokinase group (50,000 IU every day for a maximum of 6 days) and the urokinase group of conventional doses (100,000 IU every day). The success of treatment is defined as clinical and radiological improvements without surgical care or reception again within 1 month.

Results: forty-four patients received half the urokinase dose, while 48 patients were treated with conventional doses. The two groups relatively suitable with the initial not including the number of higher serum blood cells and the higher prevalence of empyema in the half-dose group. The level of success of care is not different between two groups (p = 0.048). There is no difference in mortality in hospitals and surgical treatments, duration of hospitalization, and duration of the catheter. In multivariate analysis, urokinase dose is not a predictor for the success of treatment.

Conclusion: Half the Urokinase intrapleural dose is equally effective with conventional doses to treat patients with CPPE or Empyema.

Can dissolve in urokinase plasminogen receptors predict results after heart surgery?

Objective: Receptor Activator Plasinogen Urokinase Soluble (Supar) is a biomarker that has been involved in several heart pathologies and has been shown to increase in critical painful populations. We measure plasma sounds in heart surgical cohorts to evaluate their ability to predict prolonged intensive care units (ICU) and hospitalization of hospitals and development of complications after surgery. We compare flares against Euroscore II and C-reactive protein (CRP).

Method: Ninety patients undergoing heart surgery are recruited with samples taken before surgery and in the postoperative days 1, 2 and 3. Supar is measured using an immunosorben test related to enzymes. The area under the recipient operator curve (AUROC) is used to test flare prediction skills. Comparisons made with Euroscore II and CRP.

Results: Super increases from time to time (p <0.001) with a higher level in patients who need an ICU and ina hospitalized hospitals, and prolonged ventilation (P <0.05). Supar predictions for prolonged ICU and live in hospitals, and prolonged ventilation at all time points (Auroc 0.66-0.74). Interestingly, this association was also observed before surgery, with pre-operative supernuming supplementing a prolonged ICU (Auroc 0.66), and lived in hospitals (Auroc 0.67) and prolonged ventilation (Auroc 0.74). Predictive value of Prooperative Supar compared to Euroscore II and CRP.

Conclusion: Sumba increases after heart surgery and higher levels on those who need a prolonged ICU live, live in a prolonged hospital and prolonged ventilation. Preender’s voice compared to Euroscore II and CRP in predictions of this result. Voice can be a useful biomarker in predicting the results after heart surgery, help inform clinical decision making.

 Fibrinolysis with Lower Dose Urokinase in Patients with Complicated Parapneumonic Effusion
Fibrinolysis with Lower Dose Urokinase in Patients with Complicated Parapneumonic Effusion

The inhibition structure of fab affinity inhibition against plasminogen activator urokinase reveals the basis of potential and specificity

U33 affinity maturation, upa recombinant fab inhibitors, used to increase affinity and inhibitory effects compared to great parents. Arginine scanning of the six U33 CDR loops was carried out to identify the determinant of initial binding since the UPA preferred to arginine in the pocket of the binding of the main substrate. Two CDR loops are chosen to make engineered affinity maturation libraries from Diversified U33 around Argl91 (CDR L3) and ARGH52 (CDR H2). Biopanning of random U33 libraries under strict conditions produce eight fabs with improving binding properties. One of the most powerful, AB2 inhibitors, shows a decrease of 13-fold in IC50 when compared to U33 is largely due to the decrease in the rate of off.

To identify the contribution of the interface residue that can undergo structural rearrangement on the interface formation we use X-ray traces and mass spectrometry (XFMS). Four residues show a decrease in solvent accessibility, and its grouping shows that AB2 targets active sites and also involves residues in the pocket adjacent to human upa. The 2.9 Å’s crystal structure of the AB2-bound to the upa shows the binding mode where the L1 CDR loop inserts into the site of cleft active and acts as a determinant of inhibition. The selectivity determinant of this binding mode is not like FAB inhibition previously identified with serine proteases related to UPA, MTSP-1, HGFA and FXIA. CDRS H2 and L3 loops help in the interface formation and provide critical salt bridges to remodel the loop around the active location of the upa that provides specificity and further evidence that antibodies can be a strong and selective proteolytic inhibitor. 931 patients were included.

Plasminogen Activator, Urokinase Receptor (uPAR) Antibody

20-abx104177
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20-abx114533
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20-abx174092
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Plasminogen Activator, Urokinase Receptor (uPAR) Antibody

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20-abx178039
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Plasminogen Activator, Urokinase Receptor (uPAR) Antibody (PE)

20-abx270884
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20-abx270965
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20-abx270652
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20-abx270733
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Plasminogen Activator, Urokinase Receptor (uPAR) Antibody (FITC)

20-abx273839
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20-abx270420
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20-abx274206
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20-abx274392
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Plasminogen Activator, Urokinase Receptor (uPAR) Antibody (FITC)

20-abx274393
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Plasminogen Activator, Urokinase Receptor (uPAR) Antibody (FITC)

abx273839-25mg 25 mg
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EUR 412.5

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EUR 325

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20-abx272664
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20-abx273147
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abx271932-1ml 1 ml
EUR 737.5

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EUR 337.5

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EUR 337.5

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EUR 229.4

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EUR 287.3

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EUR 217.9

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EUR 272.2

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EUR 212.2

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EUR 265.4

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EUR 212.2

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CAU28479-200ul 200ul
EUR 265.4

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EUR 212.2

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CAU28480-200ul 200ul
EUR 265.4

Plasminogen Activator, Urokinase Receptor (uPAR) Monoclonal Antibody

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EUR 242.9

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EUR 304.1

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EUR 179

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EUR 225.6

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EUR 282.2

Monoclonal Antibody to Plasminogen Activator, Urokinase Receptor (uPAR)

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EUR 257

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EUR 221

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EUR 221

Polyclonal Antibody to Plasminogen Activator, Urokinase Receptor (uPAR)

PAA141Hu03 100ul
EUR 221

Polyclonal Antibody to Plasminogen Activator, Urokinase Receptor (uPAR)

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EUR 228

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EUR 227

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EUR 239

Polyclonal Antibody to Plasminogen Activator, Urokinase Receptor (uPAR)

MBS2002981-01mL 0.1mL
EUR 170

Polyclonal Antibody to Plasminogen Activator, Urokinase Receptor (uPAR)

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EUR 215

Polyclonal Antibody to Plasminogen Activator, Urokinase Receptor (uPAR)

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EUR 350

Polyclonal Antibody to Plasminogen Activator, Urokinase Receptor (uPAR)

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EUR 425

Polyclonal Antibody to Plasminogen Activator, Urokinase Receptor (uPAR)

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EUR 1160

Polyclonal Antibody to Plasminogen Activator, Urokinase Receptor (uPAR)

MBS2004184-01mL 0.1mL
EUR 165

Polyclonal Antibody to Plasminogen Activator, Urokinase Receptor (uPAR)

MBS2004184-02mL 0.2mL
EUR 200

Polyclonal Antibody to Plasminogen Activator, Urokinase Receptor (uPAR)

MBS2004184-05mL 0.5mL
EUR 325

Polyclonal Antibody to Plasminogen Activator, Urokinase Receptor (uPAR)

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EUR 395

Polyclonal Antibody to Plasminogen Activator, Urokinase Receptor (uPAR)

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EUR 1075

Polyclonal Antibody to Plasminogen Activator, Urokinase Receptor (uPAR)

MBS2004920-01mL 0.1mL
EUR 170

Polyclonal Antibody to Plasminogen Activator, Urokinase Receptor (uPAR)

MBS2004920-02mL 0.2mL
EUR 205

Polyclonal Antibody to Plasminogen Activator, Urokinase Receptor (uPAR)

MBS2004920-05mL 0.5mL
EUR 330

Polyclonal Antibody to Plasminogen Activator, Urokinase Receptor (uPAR)

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EUR 405

Polyclonal Antibody to Plasminogen Activator, Urokinase Receptor (uPAR)

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EUR 1100

Monoclonal Antibody to Plasminogen Activator, Urokinase Receptor (uPAR)

MBS2085823-INQUIRE INQUIRE Ask for price

Monoclonal Antibody to Plasminogen Activator, Urokinase Receptor (uPAR)

MBS2111526-INQUIRE INQUIRE Ask for price

Polyclonal Antibody to Plasminogen Activator, Urokinase Receptor (uPAR)

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EUR 165

Polyclonal Antibody to Plasminogen Activator, Urokinase Receptor (uPAR)

MBS2005014-02mL 0.2mL
EUR 200

Polyclonal Antibody to Plasminogen Activator, Urokinase Receptor (uPAR)

MBS2005014-05mL 0.5mL
EUR 325

Polyclonal Antibody to Plasminogen Activator, Urokinase Receptor (uPAR)

MBS2005014-1mL 1mL
EUR 395

Polyclonal Antibody to Plasminogen Activator, Urokinase Receptor (uPAR)

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EUR 1075

Polyclonal Antibody to Plasminogen Activator, Urokinase Receptor (uPAR)

MBS2003427-01mL 0.1mL
EUR 165

Polyclonal Antibody to Plasminogen Activator, Urokinase Receptor (uPAR)

MBS2003427-02mL 0.2mL
EUR 200

Polyclonal Antibody to Plasminogen Activator, Urokinase Receptor (uPAR)

MBS2003427-05mL 0.5mL
EUR 325

Polyclonal Antibody to Plasminogen Activator, Urokinase Receptor (uPAR)

MBS2003427-1mL 1mL
EUR 395

Polyclonal Antibody to Plasminogen Activator, Urokinase Receptor (uPAR)

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EUR 1075

Plasminogen Activator, Urokinase Receptor (uPAR) Monoclonal Antibody (Rat)

4-MAA141Ra21
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Description: A Mouse monoclonal antibody against Rat Plasminogen Activator, Urokinase Receptor (uPAR)

Plasminogen Activator, Urokinase Receptor (uPAR) Polyclonal Antibody (Rat)

4-PAA141Ra01
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Description: A Rabbit polyclonal antibody against Rat Plasminogen Activator, Urokinase Receptor (uPAR)

Plasminogen Activator, Urokinase Receptor (uPAR) Monoclonal Antibody (Human)

4-MAA141Hu21
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Description: A Mouse monoclonal antibody against Human Plasminogen Activator, Urokinase Receptor (uPAR)

Plasminogen Activator, Urokinase Receptor (uPAR) Polyclonal Antibody (Human)

4-PAA141Hu01
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Description: A Rabbit polyclonal antibody against Human Plasminogen Activator, Urokinase Receptor (uPAR)

Plasminogen Activator, Urokinase Receptor (uPAR) Polyclonal Antibody (Human)

4-PAA141Hu02
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Description: A Rabbit polyclonal antibody against Human Plasminogen Activator, Urokinase Receptor (uPAR)

Plasminogen Activator, Urokinase Receptor (uPAR) Polyclonal Antibody (Human)

4-PAA141Hu03
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Description: A Rabbit polyclonal antibody against Human Plasminogen Activator, Urokinase Receptor (uPAR)

Plasminogen Activator, Urokinase Receptor (uPAR) Polyclonal Antibody (Mouse)

4-PAA141Mu01
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Description: A Rabbit polyclonal antibody against Mouse Plasminogen Activator, Urokinase Receptor (uPAR)

Kaplan and COX proportional hazard analysis is used to explore the potential relationship between the value of Supar and pre-operating HSCRP and all causes of death up to one year after surgery. Thirty days of death are predicted from flares, HSCRP, and Euroscore II with logistic regression and comparing using areas under the recipient’s operating characteristics curve and brier score. After adjustment for known persons, doubling Super and HSCRP according to the danger ratio for all causes of death 2.27.

Dustin

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