Objective: Cervical cancer metastasis results in a poor prognosis and increased mortality, which is not free from the accumulation of inflammatory reaction by prostaglandin E2 (PGE2). As a special G-protein coupled receptors of PGE2, EP3 is shown as a negative prognosticator cervical malignancy. Now, we aim to investigate the pathological mechanism of EP3 in modulating carcinogenesis of cervical cancer.
Methods: Bioinformatics analysis was used to identify PAI-1 and Upar correlation with EP3 expression, as well as the prognosis of patients with cervical cancer. In vitro analyzes were conducted to investigate the role of EP3 against cervical cancer proliferation and migration.
Results: In vitro studies indicate that sulprostone (EP3 agonist) increases the proliferation and migration of cervical cancer cells, whereas silencing of EP3 inhibits their proliferation and migration. Furthermore, EP3 knockdown increased the expression of plasminogen activator inhibitor type 1 (PAI-1), urokinase-type plasminogen activator receptor (Upar), and phosphorylated extracellular signal-regulated kinase 1/2 (p-ERK1 / 2), but decreased p53 expression. bioinformatics analysis showed that both PAI-1 and Upar correlated with EP3 expression, as well as the prognosis of patients with cervical cancer. Survival analysis further indicated that excessive Upar (IRSā„2) correlated with overall survival rates lower than patients with advanced cervical cancer (FIGO III-IV).
Conclusion: These results indicate that the EP3 signaling pathway may facilitate migration of cervical cancer cells through modulation of the expression of Upar. Therefore, EP3 and Upar could represent a new therapeutic target in the treatment of cervical cancer in stage wins.
Prostaglandin E2 receptor 3 (EP3) signaling promotes migration of cervical cancer via urokinase-type plasminogen activator receptor (uPAR)
Intrapleural urokinase directly under medical thoracoscopy for the diagnosis of TB pleurisy.
medical thoracoscopy, also called “local anesthetic thoracoscopy” and “pleuroscopy,” is a single-port endoscopic minimally invasive technique that provides direct visualization of the pleural surface and channel to perform diagnostic and therapeutic procedures. However, this technique does not help when there are substantial fibrous adhesions. We report the first case of intrapleural urokinase directly under medical thoracoscopy for the diagnosis of malignant pleural mesothelioma with pleural effusion severe multiloculated in 2019. This is the second report on the efficacy of intrapleural urokinase directly under medical thoracoscopy for the diagnosis of pleural effusion multiloculated. Urokinase-induced intrapleural fibrinolysis, which removed the fibrous septa, consequently increasing the field of view under the endoscope within only 10 minutes. fibrinolytic effect appears very quickly.
This technique is available for tuberculous pleurisy with pleural effusion multiloculated severe.
Given the accumulating evidence on the negative predictive value of soluble urokinase plasminogen activator receptor (Supar), a group of experts from the fields of intensive care medicine, emergency medicine, internal medicine and infectious diseases at the position statement framing Supar role in screening patients admitted to the emergency department. The statement was framed taking into account the existing publications and research experience of our own.
Description: A competitive ELISA for quantitative measurement of Canine Urokinase plasminogen activator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Urokinase plasminogen activator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Urokinase plasminogen activator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
*Mouse Urokinase (uPA) Active Recombinant Low Molecular Weight
Description: A competitive ELISA for quantitative measurement of Canine Urokinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Urokinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Urokinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Dog Urokinase type Plasminogen Activator Receptor ELISA kit
Description: A sandwich ELISA for quantitative measurement of Canine Urokinase type Plasminogen Activator Receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Dog Urokinase type Plasminogen Activator Receptor ELISA kit
Description: A sandwich ELISA for quantitative measurement of Canine Urokinase type Plasminogen Activator Receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Dog Urokinase type Plasminogen Activator Receptor ELISA kit
Description: A sandwich ELISA for quantitative measurement of Canine Urokinase type Plasminogen Activator Receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Dog soluble urokinase plasminogen activator receptor ELISA kit
Description: A sandwich ELISA for quantitative measurement of Canine soluble urokinase plasminogen activator receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Dog soluble urokinase plasminogen activator receptor ELISA kit
Description: A sandwich ELISA for quantitative measurement of Canine soluble urokinase plasminogen activator receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Dog soluble urokinase plasminogen activator receptor ELISA kit
Description: A sandwich ELISA for quantitative measurement of Canine soluble urokinase plasminogen activator receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Dog suPAR(soluble Plasminogen Activator, Urokinase Receptor) ELISA Kit
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog suPAR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog suPAR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog suPAR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm Ā± 10nm. The concentration of Dog suPAR in the samples is then determined by comparing the OD of the samples to the standard curve.
Dog suPAR(soluble Plasminogen Activator, Urokinase Receptor) ELISA Kit
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog suPAR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog suPAR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog suPAR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm Ā± 10nm. The concentration of Dog suPAR in the samples is then determined by comparing the OD of the samples to the standard curve.
Description: A competitive ELISA for quantitative measurement of Rat Urokinase plasminogen activator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Urokinase plasminogen activator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Urokinase plasminogen activator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Urokinase plasminogen activator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Urokinase plasminogen activator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Urokinase plasminogen activator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Urokinase plasminogen activator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Urokinase plasminogen activator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Urokinase plasminogen activator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Urokinase plasminogen activator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Urokinase plasminogen activator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Mouse Urokinase plasminogen activator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Urokinase plasminogen activator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Urokinase plasminogen activator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Urokinase plasminogen activator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rabbit Urokinase plasminogen activator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rabbit Urokinase plasminogen activator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rabbit Urokinase plasminogen activator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey Urokinase plasminogen activator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey Urokinase plasminogen activator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Monkey Urokinase plasminogen activator in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich quantitative ELISA assay kit for detection of Rat Plasminogen Activator, Urokinase (uPA) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Rat Plasminogen Activator, Urokinase (uPA) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Rat Plasminogen Activator, Urokinase (uPA) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Rat urokinase plasminogen activator, uPA ELISA kit
Description: Quantitativesandwich ELISA kit for measuring Rat urokinase plasminogen activator, uPA in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Rat urokinase plasminogen activator, uPA ELISA kit
Description: Quantitativesandwich ELISA kit for measuring Rat urokinase plasminogen activator, uPA in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Plasminogen Activator, Urokinase (uPA) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Plasminogen Activator, Urokinase (uPA) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Plasminogen Activator, Urokinase (uPA) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Pig Plasminogen Activator, Urokinase (uPA) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Pig Plasminogen Activator, Urokinase (uPA) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.
Rat Plasminogen Activator, Urokinase (uPA) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Plasminogen Activator, Urokinase (uPA) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Rat Plasminogen Activator, Urokinase (uPA) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Plasminogen Activator, Urokinase (uPA) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Rat Plasminogen Activator, Urokinase (uPA) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Plasminogen Activator, Urokinase (uPA) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Rat Plasminogen Activator, Urokinase (uPA) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Plasminogen Activator, Urokinase (uPA) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Rat Plasminogen Activator, Urokinase (uPA) ELISA Kit
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Rat Plasminogen Activator, Urokinase (uPA) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig uPA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig uPA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig uPA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm Ā± 10nm. The concentration of Pig uPA in the samples is then determined by comparing the OD of the samples to the standard curve.
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig uPA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig uPA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig uPA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm Ā± 10nm. The concentration of Pig uPA in the samples is then determined by comparing the OD of the samples to the standard curve.
Rat uPA(Plasminogen Activator, Urokinase) ELISA Kit
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat uPA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat uPA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat uPA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm Ā± 10nm. The concentration of Rat uPA in the samples is then determined by comparing the OD of the samples to the standard curve.
Rat uPA(Plasminogen Activator, Urokinase) ELISA Kit
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat uPA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat uPA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat uPA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm Ā± 10nm. The concentration of Rat uPA in the samples is then determined by comparing the OD of the samples to the standard curve.
The main contents of this statement is that Supar is a non-specific marker associated with high negative predictive value for an unfavorable outcome; levels <4 ng / ml indicates that it is safe to discharge patients, while levels> 6 ng / ml is a worrying sign for the risk of an unfavorable outcome. However, the level of Supar should always be interpreted in the light of the patient’s history.
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