Natural killer cells inhibit breast cancer cell invasion through downregulation of urokinase‑type plasminogen activator

Natural killer cells inhibit breast cancer cell invasion through downregulation of urokinase‑type plasminogen activator

Triple-negative breast cancer (TNBC) is one of the most aggressive types of breast cancer, and there is no effective therapeutic target until now. Natural cell killer (NK) is functionally a variety of lymphocytes that recognize and kill cancer cells. Although it is clear that cells have mobilized antitumor activity in the micro tumor environment, their role in the aggressive development of TNBC has not been explained in detail. In this study, we investigated the effect of NK cells in MDA-MB-231 TNBC cells using indirect coquesting systems. The invasive phenotype of MDA-MB-231 cells was significantly blocked by co-culture with NK cells. In particular, the expression of urokinase type plasminogen activator (UPA) is greatly reduced by NK cells.

Analysis of cytokine arrays shows that the level of interleukin (IL) -10, IL-6, IL-8, C-C (CCL) motif ligands (CCL) 5, and CCL2 increases in the condition of the condition of co-cultivation cells. Among these cytokines, IL-6 plays an important role in downregulation and inhibition of UPAs induced by NK cells from invasive phenotypes of MDA-MB-231 cells and HS578T cells. We analyzed the interactive analysis of the interactive analysis of gene expression protection for correlation between IL-6 and UPA with the survival of breast cancer patients as a whole.

Kaplan-Meier Survival Analysis reveals that the low IL-6 / UPA ratio is associated with the survival of poor breast cancer patients, showing it as an important factor to determine the survival of overall breast cancer patients. Taken together, our findings show that NK cells in the tumor environment inhibit the invasion of TNBC cells through the inhibition of UPA mediated by IL-6.

Prediction model for relapse of chronic subdural hematoma in patients undergoing a craniostomy twist-drill combined with urokinase instillation

Repetition of chronic subdural hematoma (CSDH) is a high post-treatment. In this study, we aim to build individual models for predicted recurrence of postoperative CSDH in patients undergoing a twist-drill cranostomy combined with urokinase (UK). In total, 183 patients with CSDH are retrospectively registered. In short, 21 candidate factors were taken from past medical records. Absolute shrinkage and regression operator selection is adopted to reduce high data dimensions. Four predictors: volume of preoperative hematoma, ensefalatrofi, re-expansion of the brain, and the frequency of British investing filtered from 21 candidate factors using the operator method of depreciation and absolute elections. The binary logistic regression model is employed to build a pre-operative and postoperative prediction model.

The pre-operative model includes the volume of preoperative hematoma and ensefalatrofi while the postoperative model includes brain re-expansion and the frequency of English instillations. Predictive performance of nomograms is evaluated by the recipient’s operating characteristics curve and the calibration chart. The area under the pre-operative and postoperative model curve was 0.755 (95% confidence interval: 0.690-0.889) and 0.782 (95% confidence interval: 0.720-0.936), respectively, showed good discrimination capabilities. The calibration results show a good installation between predicted probabilities and actual probabilities.

Finally, the decision curve analysis reveals excellent clinical performance of the proposed nomogram. Functionally, pre-operative models are used to identify high-risk patients with CSDH and the application of Britain, while postoperative models are applied to guide the communication of doctors during the follow-up period. 2 This prediction model provides a basis for further clinical and experimental studies.

 Natural killer cells inhibit breast cancer cell invasion through downregulation of urokinase‑type plasminogen activator
Natural killer cells inhibit breast cancer cell invasion through downregulation of urokinase‑type plasminogen activator

Camp-ERK1 / 2 signaling path set prolifer granulose cell granulose activator plasminogen urokinase

Despite the urokinase type plasminogen activator (Plau) and urokinase type Plasminogen (PLAUR) activator receptors have been reported to play a major role in the ovarian function, the right contribution to the development of follicular mammals is still unclear. In this study, we first observed that Plau and Plas were present in granulose cells Bovine (GCS). Following the culture of granulose cells with Plau (0.5 ng / mL) and PLAUR antibodies (10 μg / ml) separately and together for 24 or 48 hours, the proliferation test shows that the interaction between Plau and PLAAs contributed to the proliferation of GC Bovine. To study the potential of the path involved in the proliferation of cells induced plaques / Plaa, Elisa and West Blotting are carried out.

We found that Pluu significantly increased the phosphorylation ratio to ERK1 / 2 non-phosphorylation through PLAUR signaling. Further treatment with U0126, certain ERK1 / 2 phosphorylation inhibitors, significantly pressing Plau / Plaa-induction phosphorylation and cell proliferation. In addition, we found that the Plu and Plau significantly increased the level of intracellular camps, and used RP-CAMP, certain PKA inhibitors, preventing plaques / plaulas from promoting the proliferation of ERK1 / 2 and GC lines.

Urokinase Antibody

abx024172-200ul 200 ul
EUR 693.6

Urokinase Antibody

GWB-444707 0.25 mg Ask for price

Urokinase Antibody

GWB-24B3D4 1 mg Ask for price

Urokinase Antibody

MBS9436184-005mL 0.05mL
EUR 325

Urokinase Antibody

MBS9436184-01mL 0.1mL
EUR 435

Urokinase Antibody

MBS9436184-5x01mL 5x0.1mL
EUR 1810

Mouse Amminoterminal Fragment Urokinase [ATF-Urokinase (uPA)] Antibody

12171-05011 150 ug
EUR 175

Mouse Urokinase (LMW)

MBS480781-005mg 0.05mg
EUR 850

Mouse Urokinase (LMW)

MBS480781-5x005mg 5x0.05mg
EUR 3615

Mouse anti Human Urokinase/PLAU Monoclonal Antibody

MBS2543476-006mL 0.06mL
EUR 250

Mouse anti Human Urokinase/PLAU Monoclonal Antibody

MBS2543476-012mL 0.12mL
EUR 370

Mouse anti Human Urokinase/PLAU Monoclonal Antibody

MBS2543476-02mL 0.2mL
EUR 550

Polyclonal Urokinase antibody

APR10695G 0.1mg
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human Urokinase . This antibody is tested and proven to work in the following applications:

Urokinase Antibody, Concentrate

PAB751C 0.2ml
EUR 465

UROKINASE Antibody (C-term)

GWB-D2CDCD 0.1 mg Ask for price

Inactivated Mouse Urokinase

MBS136020-005mg 0.05mg
EUR 880

Inactivated Mouse Urokinase

MBS136020-01mg 0.1mg
EUR 1380

Inactivated Mouse Urokinase

MBS136020-5x01mg 5x0.1mg
EUR 5985

Mouse Urokinase ELISA kit

E01A26014 96T
EUR 700
Description: ELISA

Mouse Urokinase ELISA kit

E03U0010-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Mouse Urokinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Urokinase ELISA kit

E03U0010-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Mouse Urokinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Urokinase ELISA kit

E03U0010-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Mouse Urokinase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Urokinase ELISA Kit

EIA06502m each
EUR 480

Mouse Urokinase ELISA Kit

MBS044609-10x96StripWells 10x96-Strip-Wells
EUR 6725

Mouse Urokinase ELISA Kit

MBS044609-48StripWells 48-Strip-Wells
EUR 550

Mouse Urokinase ELISA Kit

MBS044609-5x96StripWells 5x96-Strip-Wells
EUR 3420

Mouse Urokinase ELISA Kit

MBS044609-96StripWells 96-Strip-Wells
EUR 765

Mouse Urokinase ELISA Kit

MBS269159-10x96StripWells 10x96-Strip-Wells
EUR 3290

Mouse Urokinase ELISA Kit

MBS269159-48StripWells 48-Strip-Wells
EUR 295

Mouse Urokinase ELISA Kit

MBS269159-5x96StripWells 5x96-Strip-Wells
EUR 1800

Mouse Urokinase ELISA Kit

MBS269159-96StripWells 96-Strip-Wells
EUR 450

Mouse Urokinase ELISA Kit

MBS733549-10x96StripWells 10x96-Strip-Wells
EUR 5685

Mouse Urokinase ELISA Kit

MBS733549-48StripWells 48-Strip-Wells
EUR 485

Mouse Urokinase ELISA Kit

MBS733549-5x96StripWells 5x96-Strip-Wells
EUR 3020

Mouse Urokinase ELISA Kit

MBS733549-96StripWells 96-Strip-Wells
EUR 690

Mouse Urokinase ELISA kit

E03U0010-48wellsplate 48 wells plate
EUR 280

Mouse Urokinase ELISA kit

E03U0010-96wellsplate 96 wells plate
EUR 405

Active Mouse Urokinase, LMW

MBS135667-005mg 0.05mg
EUR 855

Active Mouse Urokinase, LMW

MBS135667-01mg 0.1mg
EUR 1335

Active Mouse Urokinase, LMW

MBS135667-5x01mg 5x0.1mg
EUR 5785

Active Mouse Urokinase, HMW

MBS135745-005mg 0.05mg
EUR 510

Active Mouse Urokinase, HMW

MBS135745-01mg 0.1mg
EUR 915

Active Mouse Urokinase, HMW

MBS135745-5x01mg 5x0.1mg
EUR 3875

Plasminogen Activator, Urokinase (uPA) Polyclonal Antibody (Mouse)

4-PAA140Mu01
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Description: A Rabbit polyclonal antibody against Mouse Plasminogen Activator, Urokinase (uPA)

Urokinase, Mouse, active (LMW)

MBS653960-005mg 0.05mg
EUR 1290

Urokinase, Mouse, active (LMW)

MBS653960-5x005mg 5x0.05mg
EUR 5580

Mouse urokinase(UK ) Elisa Kit

EK730357 96 Wells
EUR 0.68

Mouse Urokinase (UK) ELISA Kit

MBS3807337-10x96StripWells 10x96-Strip-Wells
EUR 6725

Mouse Urokinase (UK) ELISA Kit

MBS3807337-48StripWells 48-Strip-Wells
EUR 550

Mouse Urokinase (UK) ELISA Kit

MBS3807337-5x96StripWells 5x96-Strip-Wells
EUR 3420

Mouse Urokinase (UK) ELISA Kit

MBS3807337-96StripWells 96-Strip-Wells
EUR 765

Mouse urokinase,UK ELISA Kit

SL0557Mo -
EUR 528

Urokinase Conjugated Antibody

C49439 100ul
EUR 476.4

Urokinase polyclonal antibody

BS91407 50ul
EUR 398
Description: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Urokinase Conjugated Antibody

MBS9457048-01mLAF350 0.1mL(AF350)
EUR 480

Urokinase Conjugated Antibody

MBS9457048-01mLAF405 0.1mL(AF405)
EUR 480

Urokinase Conjugated Antibody

MBS9457048-01mLAF488 0.1mL(AF488)
EUR 480

Urokinase Conjugated Antibody

MBS9457048-01mLAF555 0.1mL(AF555)
EUR 480

Urokinase Conjugated Antibody

MBS9457048-01mLBiotin 0.1mL(Biotin)
EUR 480

Rat Urokinase (uPA) Antibody

12172-05011 150 ug
EUR 175

Plasminogen Activator, Urokinase (uPA) Polyclonal Antibody (Mouse), PE

4-PAA140Mu01-PE
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Description: A Rabbit polyclonal antibody against Mouse Plasminogen Activator, Urokinase (uPA). This antibody is labeled with PE.

Mouse Amminoterminal Fragment Urokinase [ATF-Urokinase (uPA)] Antibody (Biotin Conjugate)

12171-05021 150 ug
EUR 229

Mouse Anti-Human Urokinase/uPA monoclonal antibody, clone NN13

CABT-ZB451 50 μl
EUR 456
Description: Mouse

Mouse Anti-Human Urokinase/uPA monoclonal antibody, clone NN16

CABT-ZB844 50 μl
EUR 456
Description: Mouse

Plasminogen Activator, Urokinase (uPA) Polyclonal Antibody (Mouse), APC

4-PAA140Mu01-APC
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Description: A Rabbit polyclonal antibody against Mouse Plasminogen Activator, Urokinase (uPA). This antibody is labeled with APC.

Plasminogen Activator, Urokinase (uPA) Polyclonal Antibody (Mouse), Cy3

4-PAA140Mu01-Cy3
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Description: A Rabbit polyclonal antibody against Mouse Plasminogen Activator, Urokinase (uPA). This antibody is labeled with Cy3.

Plasminogen Activator, Urokinase (uPA) Polyclonal Antibody (Mouse), FITC

4-PAA140Mu01-FITC
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Description: A Rabbit polyclonal antibody against Mouse Plasminogen Activator, Urokinase (uPA). This antibody is labeled with FITC.

Plasminogen Activator, Urokinase (uPA) Polyclonal Antibody (Mouse), HRP

4-PAA140Mu01-HRP
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Description: A Rabbit polyclonal antibody against Mouse Plasminogen Activator, Urokinase (uPA). This antibody is labeled with HRP.

Human Urokinase (uPA) Antibody

11651-05011 150 ug
EUR 175

*Mouse Urokinase (uPA) HIS Labeled

IMSUPAHIS100UG each
EUR 635
Description: *Mouse Urokinase (uPA) HIS Labeled

*Mouse Urokinase (uPA) HIS Labeled

IMSUPAHIS1MG each
EUR 4796
Description: *Mouse Urokinase (uPA) HIS Labeled

Mouse urokinase-UK-ELISA Kit

QY-E21572 96T
EUR 400

Plasminogen Activator, Urokinase Receptor (uPAR) Polyclonal Antibody (Mouse)

4-PAA141Mu01
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Description: A Rabbit polyclonal antibody against Mouse Plasminogen Activator, Urokinase Receptor (uPAR)

Plasminogen Activator, Urokinase (uPA) Polyclonal Antibody (Mouse), APC-Cy7

4-PAA140Mu01-APC-Cy7
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Description: A Rabbit polyclonal antibody against Mouse Plasminogen Activator, Urokinase (uPA). This antibody is labeled with APC-Cy7.

Urokinase (PLAU) Antibody (N-term)

E45R10806G-1 100 ul
EUR 395

Urokinase (PLAU) Antibody (N-term)

E45R10806G-4 50 ul
EUR 295

Urokinase (PLAU) Antibody (C-term)

MBS9210726-008mL 0.08mL
EUR 210

Urokinase (PLAU) Antibody (C-term)

MBS9210726-04mL 0.4mL
EUR 430

Urokinase (PLAU) Antibody (C-term)

MBS9210726-5x04mL 5x0.4mL
EUR 1910

Urokinase (PLAU) Antibody (N-term)

MBS9202443-008mL 0.08mL
EUR 210

Urokinase (PLAU) Antibody (N-term)

MBS9202443-04mL 0.4mL
EUR 430

Urokinase (PLAU) Antibody (N-term)

MBS9202443-5x04mL 5x0.4mL
EUR 1910

Plasminogen Activator, Urokinase (uPA) Polyclonal Antibody (Mouse), Biotinylated

4-PAA140Mu01-Biotin
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Description: A Rabbit polyclonal antibody against Mouse Plasminogen Activator, Urokinase (uPA). This antibody is labeled with Biotin.

335 Mouse anti-human urokinase monoclonal antibody, clone PGM2005 1 ml

335 1 ml
EUR 382.8

Urokinase Rabbit monoclonal antibody

BS40454 50ul
EUR 298
Description: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Plasminogen Activator, Urokinase Receptor (uPAR) Polyclonal Antibody (Mouse), APC

4-PAA141Mu01-APC
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Description: A Rabbit polyclonal antibody against Mouse Plasminogen Activator, Urokinase Receptor (uPAR). This antibody is labeled with APC.

Plasminogen Activator, Urokinase Receptor (uPAR) Polyclonal Antibody (Mouse), Cy3

4-PAA141Mu01-Cy3
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Description: A Rabbit polyclonal antibody against Mouse Plasminogen Activator, Urokinase Receptor (uPAR). This antibody is labeled with Cy3.

Plasminogen Activator, Urokinase Receptor (uPAR) Polyclonal Antibody (Mouse), HRP

4-PAA141Mu01-HRP
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  • 20ul
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Description: A Rabbit polyclonal antibody against Mouse Plasminogen Activator, Urokinase Receptor (uPAR). This antibody is labeled with HRP.

Plasminogen Activator, Urokinase Receptor (uPAR) Polyclonal Antibody (Mouse), PE

4-PAA141Mu01-PE
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Description: A Rabbit polyclonal antibody against Mouse Plasminogen Activator, Urokinase Receptor (uPAR). This antibody is labeled with PE.

Mouse Plasminogen Activator, Urokinase (uPA) Antibody Pair Kit (with Standard)

MBS2098515-10x96Tests 10x96Tests
EUR 1375

Mouse Plasminogen Activator, Urokinase (uPA) Antibody Pair Kit (with Standard)

MBS2098515-10x96TestsMBS2090685AbPairsSupportPack110x96Tests 10x96Tests+MBS2090685(AbPairsSupportPack1,10x96Tests)
EUR 1500

Mouse Plasminogen Activator, Urokinase (uPA) Antibody Pair Kit (with Standard)

MBS2098515-5x96Tests 5x96Tests
EUR 875

Mouse Plasminogen Activator, Urokinase (uPA) Antibody Pair Kit (with Standard)

MBS2098515-5x96TestsMBS2090685AbPairsSupportPack15x96Tests 5x96Tests+MBS2090685(AbPairsSupportPack1,5x96Tests)
EUR 950

*Mouse Total Urokinase (uPA) ELISA Kit

IMSUPAKTT each
EUR 528
Description: *Mouse Total Urokinase (uPA) ELISA Kit

*Mouse Active Urokinase (uPA) ELISA Kit

IMSUPAKTA each
EUR 528
Description: *Mouse Active Urokinase (uPA) ELISA Kit

Plasminogen Activator, Urokinase Receptor (uPAR) Polyclonal Antibody (Mouse), FITC

4-PAA141Mu01-FITC
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Description: A Rabbit polyclonal antibody against Mouse Plasminogen Activator, Urokinase Receptor (uPAR). This antibody is labeled with FITC.

*Mouse Urokinase (uPA) Recombinant Inactivated

IMSUPARIN100UG each
EUR 1047
Description: *Mouse Urokinase (uPA) Recombinant Inactivated

*Mouse Urokinase (uPA) Recombinant Inactivated

IMSUPARIN50UG each
EUR 635
Description: *Mouse Urokinase (uPA) Recombinant Inactivated

Anti-PLAU/Urokinase Picoband antibody

MBS1750868-01mg 0.1mg
EUR 450

Anti-PLAU/Urokinase Picoband antibody

MBS1750868-5x01mg 5x0.1mg
EUR 1870

Human Urokinase/uPA Antibody Pair Set

ABPR-ZB025 5 plates, 15 plates Ask for price
Description: Quantitative determination of Human Urokinase/uPA

Urokinase (4A13) Rabbit Monoclonal Antibody

E28M5133 100ul
EUR 295

Urokinase Plasminogen Activator, (HMW), Recombinant, Mouse (uPA, U-plasminogen Activator, urokinase-type plasminogen activator, Urokinase-type plasminogen activator)

MBS638074-005mg 0.05mg
EUR 855

Urokinase Plasminogen Activator, (HMW), Recombinant, Mouse (uPA, U-plasminogen Activator, urokinase-type plasminogen activator, Urokinase-type plasminogen activator)

MBS638074-5x005mg 5x0.05mg
EUR 3630

Plasminogen Activator, Urokinase Receptor (uPAR) Polyclonal Antibody (Mouse), Biotinylated

4-PAA141Mu01-Biotin
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Description: A Rabbit polyclonal antibody against Mouse Plasminogen Activator, Urokinase Receptor (uPAR). This antibody is labeled with Biotin.

Plasminogen Activator, Urokinase Receptor (uPAR) Polyclonal Antibody (Mouse), APC-Cy7

4-PAA141Mu01-APC-Cy7
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Description: A Rabbit polyclonal antibody against Mouse Plasminogen Activator, Urokinase Receptor (uPAR). This antibody is labeled with APC-Cy7.

Plasminogen Activator, Urokinase (uPA) Antibody

abx110339-100l 100 µl
EUR 162.5

Plasminogen Activator, Urokinase (uPA) Antibody

20-abx110339
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Plasminogen Activator, Urokinase (uPA) Antibody

abx129610-100l 100 µl
EUR 262.5

Plasminogen Activator, Urokinase (uPA) Antibody

20-abx129610
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Plasminogen Activator, Urokinase (uPA) Antibody

abx129610-1ml 1 ml
EUR 687.5

Plasminogen Activator, Urokinase (uPA) Antibody

abx129610-200l 200 µl
EUR 325

Plasminogen Activator, Urokinase (uPA) Antibody

abx130234-100l 100 µl
EUR 262.5

Plasminogen Activator, Urokinase (uPA) Antibody

20-abx130234
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Plasminogen Activator, Urokinase (uPA) Antibody

abx130234-1ml 1 ml
EUR 725

Plasminogen Activator, Urokinase (uPA) Antibody

abx130234-200l 200 µl
EUR 337.5

Plasminogen Activator, Urokinase (uPA) Antibody

20-abx178032
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  • 200 ug
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Plasminogen Activator, Urokinase (uPA) Antibody

abx178032-96tests 96 tests
EUR 237.5

Plasminogen Activator, Urokinase (uPA) Antibody

20-abx178033
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  • 200 ug
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Plasminogen Activator, Urokinase (uPA) Antibody

20-abx178034
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  • 200 ug
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Plasminogen Activator, Urokinase (uPA) Antibody

20-abx178036
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  • 200 ug
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Plasminogen Activator, Urokinase (uPA) Antibody

20-abx174088
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  • 200 ug
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Plasminogen Activator, Urokinase (uPA) Antibody

abx174089-100l 100 µl
EUR 775

Plasminogen Activator, Urokinase (uPA) Antibody

20-abx174089
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  • 200 ug
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Therefore, the interaction between Plau and PLAA can be involved in accumulating camp signals and allowing activation of MAPK / ERK1 / 2, which affects GC proliferation. Here, we provide new mechanistic insights into the role of Plau and Plaa on the promotion of the Bovine GC proliferation. The findings that the potential cross-points between intracellular signals induced plaques / PLAAur affect GC’s proliferation will help in understanding the mechanisms that regulate the development of early follicular.

Dustin

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