12 Quantichrom Phasphate Assay Kit

Description: For quantitative determination of hydroxyproline and collagen in biologic and cosmetic samples. Key Features: Safe. Our hydroxyproline assay uses an improved perchlorate-free chemistry. Sensitive and accurate. Uses 20 µL sample. Linear detection range 0.5 µg/mL to 50 µg/mL hydroxyproline in 96-well plate assay. Convenient. Collagen can be hydrolyzed in Eppendorf tubes, and final incubation can be performed at 37°C. Method: OD560nm. Samples: Hydroxyproline and collagen in biologic and cosmetic samples. Species: All. Procedure: Assay takes Approx. 2 hrs. Kit size: 100 Tests. Detection limit: 0.5 µg/mL.

Description: For quantitative determination of bilirubin and evaluation of drug effects on bilirubin metabolism. Key Features: Sensitive and accurate. Detection limit 0.16 mg/dL bilirubin in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 10 min. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day. Method: OD530nm. Samples: Serum. Species: All. Procedure: Assay takes 10 min. Kit size: 180 tests. Detection limit: 0.16 mg/dL.

Description: For quantitative determination of calcium ion Ca²⁺ and evaluation of drug effects on calcium metabolism. Key Features: Sensitive and accurate. Use as little as 5 µL samples. Linear detection range 0.08 mg/dL (20 µM) to 20 mg/dL (5 mM) Ca²⁺ in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 3 min. Can be readily automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay. Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on raw biological samples i.e., in the presence of lipid, protein and minerals such as magnesium, iron and zinc. Method: OD612nm. Samples: Biological, food and environment. Species: All. Procedure: Assay takes 3 min. Kit size: 500 tests. Detection limit: 0.08 mg/dL (20 µM).

Description: For quantitative determination of chloride ion and evaluation of drug effects on chloride metabolism. Key Features: Sensitive and accurate. Use as little as 5 µL samples. Linear detection range 0.7 mg/dL (0.2 mM) to 35 mg/dL (10 mM) Cl^- in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 5 min. Can be readily automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay. Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on raw biological samples i.e., in the presence of lipid, protein and minerals such as magnesium, iron and zinc. Method: OD610nm. Samples: Biological, food and environment. Species: All. Procedure: Assay takes 5 min. Kit size: 250 tests. Detection limit: 0.7 mg/dL (0.2 mM).

Description: For quantitative determination of creatinine and evaluation of drug effects on creatinine metabolism. Key Features: Sensitive and accurate. Use 30 µL samples. Detection limit 0.10 mg/dL (8 µM) creatinine in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 5 min. Can be automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Assays can be executed in 96-well plate or cuvet. Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on raw biological samples. Method: OD510nm. Samples: Urine, serum, plasma and biological preparations. Species: All. Procedure: Assay takes 20 min. Kit size: 500 tests. Detection limit: 0.1 mg/dL (8 µM).

Description: For quantitative determination of copper(II) ion and evaluation of drug effects on Cu metabolism. Key Features: Sensitive and accurate. Linear detection range 7 µg/dL (1.0 µM) to 300 µg/dL (47 µM) copper in 96-well plate assay. Simple and high-throughput. The simple procedure can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay. Method: OD359nm. Samples: Biological, environment, food and beverage. Species: All. Procedure: Assay takes 10 min. Kit size: 250 tests. Detection limit: 7 µg/dL (1.0 µM).

Description: For quantitative determination of indican (indoxyl sulfate) in urine samples. Key Features: Fast and sensitive. Linear detection range: 0.2 - 20 mg/dL (8-800 µmoles/L). Fast and high-throughput. Homogeneous "mix-incubate-measure" type assay. Assay takes only 10 min. Can be readily automated for processing thousands of samples per day. Method: OD480nm. Samples: Urine. Species: All. Procedure: Assay takes 10 min. Kit size: 1000 tests. Detection limit: 0.2 mg/dL.

Description: For quantitative determination of peroxide and evaluation of drug effects on peroxide metabolism. Key Features: Sensitive and accurate. Enhanced color intensity using sorbitol. Detection range 0.2 µM (7 ng/mL) to 30 µM (1,020 ng/mL) H₂O₂ in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single detection reagent and incubation for 30 min. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day. Method: OD585nm. Samples: Serum, citrate-plasma, urine, cell lysate, culture media etc. Species: All. Procedure: Assay takes 30 min. Kit size: 250 tests. Detection limit: 0.2 µM.

Description: For quantitative determination of carbonyl groups or protein carbonyls and evaluation of drug modulators. Key Features: Fast and sensitive. Use of 100 µL sample. Linear detection range from 12 to 250 µM carbonyl in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the absorbance after 30 min. High-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD375nm. Samples: Chemicals with carbonyl groups (e.g. ketones, aldehydes) or protein carbonyls in biological samples (e.g. oxidized BSA, etc). Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 12 µM.

Description: For quantitative determination of chromium Cr(VI). Key Features: Sensitive and accurate. Linear detection range of 20 - 2000 µg/L Chromium. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Can be readily automated for processing thousands of samples per day. Method: OD480nm. Samples: Biological (serum, plasma etc), environmental (water, soil etc), food and beverage samples. Species: All. Procedure: Assay takes 20 min. Kit size: 250 tests. Detection limit: 20 µg/L (0.38 µM, 0.02 ppm).

Description: For quantitative determination of urea and evaluation of drug effects on urea metabolism. Key Features: Sensitive and accurate. Use 5 µL samples. Linear detection range 0.08 mg/dL (13 µM) to 100 mg/dL (17 mM) urea in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 20 min. Can be readily automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay. Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on raw biological samples i.e., in the presence of lipid and protein. Method: OD520nm (Chemical). Samples: Serum, plasma, urine, milk, cell/tissue culture, bronchoalveolar lavage (BAL), food, beverage and environment. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.08 mg/dL (13 µM).

Description: For quantitative determination of pectinase activity determination in biological samples. Key Features: Linear detection range from 9.2-100 U/L pectinase activity in a 96-well plate assay. Simple and convenient 40 minute "Add-Mix-Measure" type assay. High-throughput format and compatible with laboratory liquid handling systems. No heating required. The kit does not use toxic materials. Refer to the previously established DNS method. Method: OD600nm. Samples: Enzyme extracts, agriculture and biological samples. Species: Plant, fungal tissues, juice, bacteria, etc. Procedure: Assay takes 40 min. Kit size: 100 tests. Detection limit: 9.2 U/L.

Description: For quantitative determination of bilirubin and evaluation of drug effects on bilirubin metabolism. Key Features: Sensitive and accurate. Detection limit 0.16 mg/dL bilirubin in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 10 min. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day. Method: OD530nm. Samples: Serum. Species: All. Procedure: Assay takes 10 min. Kit size: 180 tests. Detection limit: 0.16 mg/dL.

Description: For quantitative determination of Mg²⁺ and evaluation of drug effects on magnesium metabolism. Key Features: Sensitive and accurate. Use as little as 5 µL sample. Linear detection range 0.1 mg/dL (41 µM) to 3 mg/dL (1.2 mM) Mg²⁺ in 96-well plate assay. Simple and high-throughput. The procedure involves addition of two reagents and measuring OD500nm. Can be readily automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay. Low interference in biological samples. Assays can be directly performed in serum and urine samples. Method: OD500nm. Samples: Biological, food and beverage. Species: All. Procedure: Assay takes 10 min. Kit size: 250 tests. Detection limit: 0.1 mg/dL (41 µM).

Description: For quantitative determination of phosphate and evaluation of drug effects on phosphate metabolism. Key Features: Sensitive and accurate. Linear detection range 0.3 µM (0.0028 mg/dL) to 50 µM (0.47 mg/dL) phosphate in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 30 min. Can be readily automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Assays can be executed in cuvet or 96-well plate. Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on raw biological samples i.e., in the presence of lipid, protein and minerals. Method: OD620nm (malachite green). Samples: Serum, urine, saliva, sweat, tissue culture, food, environment etc. Species: All. Procedure: Assay takes 30 min. Kit size: 500 tests. Detection limit: 3 µg/dL (0.3 µM).

Description: For quantitative determination of detergents in e.g. water and soil samples. Key Features: Sensitive and accurate. Use 20 µL sample. Linear detection range: Tween 80: 0.012 to 4 mM; Tween 20: 0.06 to 5 mM; Triton X-100: 0.23 to 12 mM; Brij L23/35: 0.09 to 5 mM; DTAC: 0.08 to 2 mM; SDS: 10 to 25 mM. Simple and convenient. The procedure involves addition of two reagents and measuring OD560nm or OD650nm. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvette or 96-well plate assay. Method: 560nm and 650nm. Samples: Biological and environmental samples. Species: All. Procedure: Assay takes 15 min. Kit size: 100 tests. Detection limit: Tween 80: 0.012 to 4 mM; Tween 20: 0.06 to 5 mM; Triton X-100: 0.23 to 12 mM; Brij L23/35: 0.09 to 5 mM; DTAC: 0.08 to 2 mM; SDS: 10 to 25 mM.

Description: For quantitative enzymatic determination of urea. Key Features: Fast and sensitive. Linear detection range (20 µL sample): 1 mg/mL (0.17 mM) to 100 mg/mL (17 mM) urea in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the absorbance after 5 minutes. Room temperature assay. No 37°C heater is needed. High-throughput. Homogeneous "mix-incubate-measure" type assay.Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD557nm. Samples: Urea in biological samples (e.g. plasma, serum, urine) and food/beverage samples (e.g. milk). Species: All. Procedure: Assay takes 5 min. Kit size: 100 tests. Detection limit: 1 mg/mL (0.17 mM).

Description: For quantitative determination of salicylate in biological samples and beauty products, mouthwash, etc. Key Features: Fast and sensitive. Linear detection range: 0.8 mM (10.9 mg/dL) to 20 mM (274.2 mg/dL) salicylate with 20 µL sample (96-well). Convenient. The procedure involves adding a single working reagent. High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well or 384-well plate assay for thousands of samples per day. Method: OD560nm. Samples: Serum, plasma, urine, food, beverage, agriculture, drugs, beauty, mouthwash samples, etc. Species: All. Procedure: Assay takes 30 min. Kit size: 100 Tests.

Description: For quantitative determination of creatinine and evaluation of drug effects on creatinine metabolism. Key Features: Sensitive and accurate. Use 30 µL samples. Detection limit 0.10 mg/dL (8 µM) creatinine in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 5 min. Can be automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Assays can be executed in 96-well plate or cuvet. Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on raw biological samples. Method: OD510nm. Samples: Urine, serum, plasma and biological preparations. Species: All. Procedure: Assay takes 20 min. Kit size: 500 tests. Detection limit: 0.1 mg/dL (8 µM).

Description: For quantitative determination of hemoglobin and evaluation of drug effects on hemoglobin metabolism. Key Features: Sensitive and accurate. Linear detection range 0.9 - 200 mg /dL hemoglobin in 96-well plate assay. Simple and high-throughput. The "mix-and-read" procedure involves addition of a single working reagent and reading the optical density. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day. Safety. Reagents do not contain any toxic components, e.g. hexacyanoferrate(III) and potassium cyanide used in Drabkin's reagent. Versatility. Assays can be executed in 96-well plate or cuvet. Method: OD400nm. Samples: Blood, serum, plasma, urine etc. Species: All. Procedure: Assay takes 5 min. Kit size: 250 tests. Detection limit: 0.9 mg/dL.

Description: For quantitative determination of peroxidase enzyme activity. Key Features: Fast and sensitive. Use as little as 10 µL samples. Linear detection range: colorimetric assays 2 to 50 U/L, fluorimetric assays 0.1 to 5 U/L peroxidase. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD570nm, or FL530/585nm. Samples: Biological. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: OD, FL: 4, 0.8 U/L.

Description: For quantitative determination of total antioxidant capacity (TAC) and evaluation of drug effects on TAC metabolism. Key Features: Sensitive and accurate. Use 20 µL sample. Linear detection range from 1.5 to 1000 µM Trolox equivalents. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 10 min. Can be readily automated as a high-throughput assay for thousands of samples per day. Method: OD570nm. Samples: Serum, plasma, urine, saliva, food and beverage etc. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: 1.5 µM.

Description: For quantitative determination of reduced glutathione (GSH) and evaluation of drug effects on glutathione metabolism. Key Features: Sensitive and accurate. Linear detection range 0.4 - 100 µM in 96-well plate. Simple and convenient. The procedure involves mixing the DTNB Reagent with sample, removing protein precipitates for proteinaceous samples, adding a second Reagent and reading the optical density. Low interference. Amino acids and common buffers do not interfere. Method: OD412nm. Samples: Whole blood, plasma, serum, urine, tissue and cell extracts. Species: All. Procedure: Assay takes 30 min. Kit size: 250 tests. Detection limit: 12 µg/dL (0.4 µM).

Description: For quantitative determination of uric acid and evaluation of drug effects on uric acid metabolism. Key Features: Sensitive and accurate. Use 5 µL samples. Linear detection range 0.22 mg/dL (13 µM) to 30 mg/dL (1785 µM) uric acid in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 30 min. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay. Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on serum samples. Method: OD590nm. Samples: Serum, plasma, urine and other biological samples. Species: All. Procedure: Assay takes 30 min. Kit size: 250 tests. Detection limit: 0.2 mg/dL (13 µM).